Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

Journal: Biological Chemistry

Article Title: Peritumoral administration of GPI-anchored TIMP-1 inhibits colon carcinoma growth in Rag-2 γ chain-deficient mice

doi: 10.1515/bc.2009.098

Figure Lengend Snippet: Figure 2 The GPI-anchored TIMP-1 suppresses proliferation of SW480 cells and inhibits tumor invasion. The effect of cell surface engineering with TIMP-1-GPI on SW480 invasion through the Matrigel basement membrane model was assessed. Optimal invasion of the SW480 cells in response to 10% FCS, 4 ng/ml EGF, or 4 ng/ml VEGF were set to ‘zero’, and the 100% inhibition value was set to the migration level observed with untreated SW480 cells (Djafarzadeh et al., 2004). 5=103 SW480 cells per 100 ml medium were cultured in 96-well microtiter plates for 24 h under standard conditions to yield firmly attached and stably growing cells (Djafarzadeh et al., 2006). The SW480 cells were then pretreated with 2, 4, 6, 8, 10, 12, and 14 ng/ml of TIMP-1-GPI or 14 ng/ml of rhTIMP-1 as described previously (Djafarzadeh et al., 2006). After 1 h the cells were washed. (A) The effect of increasing levels of TIMP-1-GPI or rhTIMP-1 control protein on the proliferation of SW480 was measured using an MTT assay (Djafarzadeh et al., 2006). MTT was added after 24 h, 48 h, or 72 h as indicated. (B) The effect on the ability of SW480 to invade Matrigel was measured using a modified Boyden chamber as described earlier (Djafarzadeh et al., 2004, 2006).

Article Snippet: The effect of TIMP-1-GPI vs. rhTIMP-1 treatment on the ability of SW480 cells to invade Matrigel was measured using induced migration to fetal calf serum (FCS) (Biocrom, Berlin, Germany), vascular endothelial growth factor (VEGF) (R&D systems, Minneapolis, USA), and EGF (Biochrom, Berlin, Germany) using a modified Boyden chamber (Djafarzadeh et al., 2004, 2006).

Techniques: Membrane, Inhibition, Migration, Cell Culture, Stable Transfection, Control, MTT Assay, Modification

Journal: Chinese Journal of Cancer Research

Article Title: Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells

doi: 10.21147/j.issn.1000-9604.2020.05.02

Figure Lengend Snippet: VEGFC and VEGFR2 protein levels in conditioned media of stable cell lines indicated by ELISA. (A) VEGFC; (B) VEGFR2. VEGF, vascular endothelial growth factor; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: ELISA was performed using the human VEGFC (cat. no. E-EL-H1600; Elabscience) and vascular endothelial growth factor receptor 2 (VEGFR2) ELISA kit (cat. no. E-EL-H1603; Elabscience), according to the manufacturer’s protocol.

Techniques: Stable Transfection, Enzyme-linked Immunosorbent Assay